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Recombinant Rabbit Monoclonal
Anti-Smad4 Rabbit mAb
Catalog Number: PTM-5449
$ 280

Clone Number: JRMR-10073-6H2L4

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF FC

Reactivity: Human, Mouse, Rat

Synonyms: DPC4, MADH4

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms DPC4, MADH4
UniProt ID

Q13485

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human
IHC-P 1:50 - 1:100 Human, Mouse, Rat
ICC/IF 1:50 Human
FC 1:50 - 1:100 Human, Mouse
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, HepG2, HEK-293
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 60 kDa
Observed band size: 60 kDa
IHC-P

Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5449

Tissue: Human kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5449

Tissue: Mouse ovary
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5449

Tissue: Rat ovary
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5449
ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-5449

Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-5449
FC

Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 90% methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat anti Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-5449

Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Secondary Ab: Goat anti Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-5449

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 280
    Recombinant Rabbit Monoclonal
    Anti-Smad4 Rabbit mAb
    Catalog #
    PTM-5054
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
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