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Anti-SMARCC1/BAF155 Rabbit pAb
Catalog Number: PTM-7011
$ 285

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155 (BAF155), SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SMARCC1, BAF155

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155 (BAF155), SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SMARCC1, BAF155
UniProt ID

Q92922

Immunogen Synthetic peptide derived from human SWI/SNF complex subunit SMARCC1
MW (kDa) 130
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 Human, Mouse
IHC-P 1:50 - 1:100 Mouse, Rat
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

SMARCC1/BAF155 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by BRG1 in vitro . SMARCC1 is an essential part of the mouse embryonic stem cell specific SWI/SNF complex (esBAF), which is necessary for early embryogenesis, especially proper brain and visceral endoderm development.

Cellular location

Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Jurkat, 2: K562, 3: Neuro-2a
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted band size: 130 kDa
Observed band size: 130 kDa
IHC-P

Tissue: mouse cerebrum
Section type: formalin-fixed & paraffinembedded section
Antigen retrieval method: high temperature and high pressure
Antigen retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counterstain: hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-7011

Tissue: mouse liver
Section type: formalin-fixed & paraffinembedded section
Antigen retrieval method: high temperature and high pressure
Antigen retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counterstain: hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-7011

Tissue: rat cerebellum
Section type: formalin-fixed & paraffinembedded section
Antigen retrieval method: high temperature and high pressure
Antigen retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counterstain: hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-7011

Tissue: rat cerebrum
Section type: formalin-fixed & paraffinembedded section
Antigen retrieval method: high temperature and high pressure
Antigen retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counterstain: hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-7011

Research Use

For research use only, not for use in diagnostic procedures.

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