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Anti-Succinyl-Histone H2B (Lys120) Rabbit pAb
Catalog Number: PTM-409
$ 420

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: H2BK120su

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2BK120su
UniProt ID

P62807

Immunogen Succinylated human histone H2B (Lys120) peptide
MW (kDa) 14
Specificity Anti-Succinyl-Histone H2B (Lys120) Rabbit pAb detects histone H2B only when it is succinylated at Lys120. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications including acetylation, phosphorylation, ubiquitylation etc. Lysine succinylation is a recently identified novel protein post-translational modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both prokaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 seconds
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa; (+) HeLa + disodium succinylate (10 mM, 4 hours)
Protein loading amount: 20 μg
Exposure time: 60 seconds
Predicted band size: 14 kDa
Observed band size: 14 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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