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Anti-Succinyl-Histone H3 (Lys14) Mouse mAb
Catalog Number: PTM-421
$ 435

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: H3K14su

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K14su
UniProt ID

P68431

Immunogen Succinylated human histone H3 (Lys14) peptide
MW (kDa) 15
Specificity Anti-Succinyl-Histone H3 (Lys14) Mouse mAb detects histone H3 only when it is succinylated at Lys14. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications including acetylation, phosphorylation, ubiquitylation etc. Lysine succinylation is a recently identified novel protein post-translational modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both prokaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody is specifically designed to facilitate studies of lysine succinylation by enabling the identification of succinylation substrates and providing insights into its functional roles.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K14 succinyl. Lane 2: H3K14 acetyl.
Lane 3: H3K14 propionyl. Lane 4: H3K14 butyryl.
Lane 5: H3K14 crotonyl. Lane 6: H3K14 unmodified.
WB

Lysates: (-): HeLa cells; (+): HeLa cells + sodium butyrate (30 mM, 4 hours) + trichostatin A (500 ng/ml, 4 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
IHC-P

Tissue: Human spleen
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-421.

Research Use

For research use only, not for use in diagnostic procedures.

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References (1)
  • HDAC1/2/3 are major histone desuccinylases critical for promoter desuccinylation
    Year 2023
    Journal Cell Discovery
    Authors Li Jialun, et al.
    Applications Unspecified
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