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Anti-Succinyllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-402
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications: IAP

Reactivity: All

Synonyms: Ksu

Product Size
500 μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Ksu
UniProt ID

/

Immunogen Succinylated lysine peptides
MW (kDa) Multiple
Specificity With the immobilization of highly specific anti-succinyllysine antibody, the Anti-Succinyllysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing succinyllysine residues, but do not cross-react with the peptides/proteins bearing other structurally similar modified residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine succinylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Lysine succinylation is an important reversible modification, and plays significant roles i nmany cellular processes. Systematical screening of lysine succinylated substrates in various cellular pathological and physiological conditions is a fundamental mission in lysine succinylation biology.To address this,the affinity chromatography with Anti-Succinyllysine Antibody Conjugated Agarose Beads followed by MS/MS identification is viewed as the most preferable and efficient method.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Succinyllysine Rabbit pAb (PTM-401) on different acylation peptides.
Peptide amount: 4 ng, 20 ng, 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided in below.
Lane 1: Succinylated peptide library. Dot 2: Malonylated peptide library.
Dot 3: Glutarylated peptide library. Dot 4: Unmodified peptide library.

Research Use

For research use only, not for use in diagnostic procedures.

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References (74)
  • Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs
    Year 2023
    Journal Microbiome
    Authors Hu Jun, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-Hydroxyisobutyrylation- and Succinylation-Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity
    Year 2023
    Journal Advanced Science
    Authors Bin Chen, et al.
    Applications Unspecified
    Reactivity
  • Role of Succinylation in Pseudorabies Virus Infection
    Year 2023
    Journal JOURNAL OF VIROLOGY
    Authors Xiaoyong Chen, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive succinylome analyses reveal that hyperthermia upregulates lysine succinylation of annexin A2 by downregulating sirtuin7 in human keratinocytes
    Year 2023
    Journal Journal of Translational Internal Medicine
    Authors Xueli Niu, et al.
    Applications Unspecified
    Reactivity
  • Global proteomic analysis reveals lysine succinylation contributes to the pathogenesis of aortic aneurysm and dissection
    Year 2023
    Journal Journal of Proteomics
    Authors Hongwei Zhang, et al.
    Applications Unspecified
    Reactivity
  • Succinyl-CoA-based energy metabolism dysfunction in chronic heart failure
    Year 2022
    Journal PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    Authors Takada Shingo, et al.
    Applications Unspecified
    Reactivity
  • Succinyl-CoA Synthetase Deficiency in Mouse Forebrain Results in Hyper-Succinylation With Perturbed Neuronal Transcriptional Regulation and Metabolism
    Year 2022
    Journal Cell Reports
    Authors Anderson Makayla Suzanne, et al.
    Applications Unspecified
    Reactivity
  • Lysine Succinylation of VBS Contributes to Sclerotia Development and Aflatoxin Biosynthesis in Aspergillus flavus
    Year 2022
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yu Wang, et al.
    Applications Unspecified
    Reactivity
  • Global proteome analyses of phosphorylation and succinylation of barley root proteins in response to phosphate starvation and recovery
    Year 2022
    Journal Frontiers in Plant Science
    Authors Juncheng Wang, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Succinyl-Proteome Profiling of Turnip (Brassica rapa var. rapa) in Response to Cadmium Stress
    Year 2022
    Journal Cells
    Authors Xiong Li, et al.
    Applications Unspecified
    Reactivity
  • Dietary Succinate Impacts the Nutritional Metabolism, Protein Succinylation and Gut Microbiota of Zebrafish
    Year 2022
    Journal Frontiers in Nutrition
    Authors Qianwen Ding, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive Profiling of Paper Mulberry (Broussonetia papyrifera) Crotonylome Reveals the Significance of Lysine Crotonylation in Young Leaves
    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Yibo Dong, et al.
    Applications Unspecified
    Reactivity
  • Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production
    Year 2022
    Journal APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
    Authors Ke Xiang, et al.
    Applications Unspecified
    Reactivity
  • Sirt5-mediated desuccinylation of OPTN protects retinal ganglion cells from autophagic flux blockade in diabetic retinopathy
    Year 2022
    Journal Cell Death Discovery
    Authors Zhang Ye, et al.
    Applications Unspecified
    Reactivity
  • Acetylome and Succinylome Profiling of Edwardsiella tarda Reveals Key Roles of Both Lysine Acylations in Bacterial Antibiotic Resistance
    Year 2022
    Journal Antibiotics-Basel
    Authors Yuying Fu, et al.
    Applications Unspecified
    Reactivity
  • The succinylome of Pinctada fucata martensii implicates lysine succinylation in the allograft-induced stress response
    Year 2022
    Journal FISH & SHELLFISH IMMUNOLOGY
    Authors Meizhen Zhang, et al.
    Applications Unspecified
    Reactivity
  • Global landscape of lysine acylomes in Bacillus subtilis
    Year 2022
    Journal Journal of Proteomics
    Authors Mingya Zhang, et al.
    Applications Unspecified
    Reactivity
  • Proteomic Quantification of Lysine Acetylation and Succinylation Profile Alterations in Lung Adenocarcinomas of Non-Smoking Females
    Year 2022
    Journal YONAGO ACTA MEDICA
    Authors Jun Wu, et al.
    Applications Unspecified
    Reactivity
  • Potential role of lysine succinylation in the response of moths to artificial light at night stress
    Year 2021
    Journal ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
    Authors Zhijuan Huang, et al.
    Applications Unspecified
    Reactivity
  • Systematic Proteome and Lysine Succinylome Analysis Reveals Enhanced Cell Migration by Hyposuccinylation in Esophageal Squamous Cell Carcinoma
    Year 2021
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Zhenchang Guo, et al.
    Applications Unspecified
    Reactivity
  • Global Insights Into Lysine Acylomes Reveal Crosstalk Between Lysine Acetylation and Succinylation in Streptomyces coelicolor Metabolic Pathways
    Year 2021
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yujiao Yang, et al.
    Applications Unspecified
    Reactivity
  • Dynamic Responses of Barley Root Succinyl-Proteome to Short-Term Phosphate Starvation and Recovery
    Year 2021
    Journal Frontiers in Plant Science
    Authors Wang Juncheng, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive Succinylome Profiling Reveals the Pivotal Role of Lysine Succinylation in Energy Metabolism and Quorum Sensing of Staphylococcus epidermidis
    Year 2021
    Journal Frontiers in Microbiology
    Authors Zhao Yiping, et al.
    Applications Unspecified
    Reactivity
  • Rhamnolipid Enhances the Nitrogen Fixation Activity of Azotobacter chroococcum by Influencing Lysine Succinylation
    Year 2021
    Journal Frontiers in Microbiology
    Authors Li Jin, et al.
    Applications Unspecified
    Reactivity
  • First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence
    Year 2021
    Journal Frontiers in Cellular and Infection Microbiology
    Authors Zeng Fuyuan, et al.
    Applications Unspecified
    Reactivity
  • The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids
    Year 2021
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Jan Frankovsky, et al.
    Applications Unspecified
    Reactivity
  • Deep Succinylproteomics of Brain Tissues from Intracerebral Hemorrhage with Inhibition of Toll-Like Receptor 4 Signaling
    Year 2021
    Journal CELLULAR AND MOLECULAR NEUROBIOLOGY
    Authors Liang Yan-Jing, et al.
    Applications Unspecified
    Reactivity
  • First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera)
    Year 2021
    Journal BMC GENOMICS
    Authors Dong Yibo, et al.
    Applications Unspecified
    Reactivity
  • Succinylation profiles of brain injury after intracerebral hemorrhage
    Year 2021
    Journal PLoS One
    Authors Yuan-Hong Deng, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive analysis of the lysine acetylome and succinylome in the hippocampus of gut microbiota-dysbiosis mice
    Year 2020
    Journal Journal of Advanced Research
    Authors Lanxiang Liu, et al.
    Applications Unspecified
    Reactivity
  • Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas comosus var. bracteatus
    Year 2020
    Journal BMC GENOMICS
    Authors Mao Meiqin, et al.
    Applications Unspecified
    Reactivity
  • Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer
    Year 2020
    Journal ONCOLOGY REPORTS
    Authors Xiuli Gao, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats
    Year 2020
    Journal International Journal of Endocrinology
    Authors Hu Baoxiang, et al.
    Applications Unspecified
    Reactivity
  • SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis
    Year 2019
    Journal FREE RADICAL BIOLOGY AND MEDICINE
    Authors Yuanzhen Ma, et al.
    Applications Unspecified
    Reactivity
  • Integrated Succinylome and Metabolome Profiling Reveals Crucial Role of S-Ribosylhomocysteine Lyase in Quorum Sensing and Metabolism of Aeromonas hydrophila*[S]
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Zujie Yao, et al.
    Applications Unspecified
    Reactivity
  • ScCobB2-mediated Lysine Desuccinylation Regulates Protein Biosynthesis and Carbon Metabolism in Streptomyces coelicolor*[S]
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Hong Zhang, et al.
    Applications Unspecified
    Reactivity
  • Phytoplasma-induced Changes in the Acetylome and Succinylome of Paulownia tomentosa Provide Evidence for Involvement of Acetylated Proteins in Witches' Broom Disease *[S]
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yabing Cao, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive Analysis of the Lysine Succinylome and Protein Co-modifications in Developing Rice Seeds *
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Xiaoxi Meng, et al.
    Applications Unspecified
    Reactivity
  • Global Proteomic Analysis Reveals Widespread Lysine Succinylation in Rice Seedlings
    Year 2019
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Kai Zhang, et al.
    Applications Unspecified
    Reactivity
  • Succinyl-proteome profiling of Pyricularia oryzae, a devastating phytopathogenic fungus that causes rice blast disease
    Year 2019
    Journal Scientific Reports
    Authors Wang Jiaoyu, et al.
    Applications Unspecified
    Reactivity
  • Quantitative succinyl-proteome profiling of Chinese hickory (Carya cathayensis) during the grafting process
    Year 2019
    Journal BMC PLANT BIOLOGY
    Authors Yuan Huwei, et al.
    Applications Unspecified
    Reactivity
  • Ammonium triggered the response mechanism of lysine crotonylome in tea plants
    Year 2019
    Journal BMC GENOMICS
    Authors Sun Jianhao, et al.
    Applications Unspecified
    Reactivity
  • Succinylome Analysis Reveals the Involvement of Lysine Succinylation in the Extreme Resistance of Deinococcus radiodurans
    Year 2019
    Journal PROTEOMICS
    Authors Congli Zhou, et al.
    Applications Unspecified
    Reactivity
  • Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis
    Year 2019
    Journal Molecular Oral Microbiology
    Authors Leng Wu, et al.
    Applications Unspecified
    Reactivity
  • Discovery and Identification of Serum Succinyl-Proteome for Postmenopausal Women with Osteoporosis and Osteopenia
    Year 2019
    Journal Orthopaedic Surgery
    Authors Li-li Zhang, et al.
    Applications Unspecified
    Reactivity
  • SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice
    Year 2018
    Journal EBioMedicine
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves
    Year 2018
    Journal PLANT CELL AND ENVIRONMENT
    Authors Heng Zhou, et al.
    Applications Unspecified
    Reactivity
  • Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002
    Year 2018
    Journal PLANT AND CELL PHYSIOLOGY
    Authors Liu Xin, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Dynamics of Proteome, Acetylome, and Succinylome during Stem-Cell Differentiation into Hepatocyte-like Cells
    Year 2018
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Zekun Liu, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Global Proteome and Lysine Succinylome Analyses Reveal the Effects of Energy Metabolism in Renal Cell Carcinoma
    Year 2018
    Journal PROTEOMICS
    Authors Naiwen Zhang, et al.
    Applications Unspecified
    Reactivity
  • Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata
    Year 2018
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Xianping Fang, et al.
    Applications Unspecified
    Reactivity
  • Quantitative proteome and lysine succinylome analyses provide insights into metabolic regulation in breast cancer
    Year 2018
    Journal Breast Cancer
    Authors Liu Chenchen, et al.
    Applications Unspecified
    Reactivity
  • Involvement of the Vitamin D Receptor in Energy Metabolism Revealed by Profiling of Lysine Succinylome of White Adipose Tissue
    Year 2017
    Journal Scientific Reports
    Authors Su Han, et al.
    Applications Unspecified
    Reactivity
  • Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer
    Year 2017
    Journal Scientific Reports
    Authors Song Yongxi, et al.
    Applications Unspecified
    Reactivity
  • Proteomic analysis of lysine succinylation of the human pathogen Histoplasma capsulatum
    Year 2017
    Journal Journal of Proteomics
    Authors Longxiang Xie, et al.
    Applications Unspecified
    Reactivity
  • Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage
    Year 2017
    Journal Journal of Proteomics
    Authors Jine Chen, et al.
    Applications Unspecified
    Reactivity
  • The first succinylome profile of Trichophyton rubrum reveals lysine succinylation on proteins involved in various key cellular processes
    Year 2017
    Journal BMC GENOMICS
    Authors Xu Xingye, et al.
    Applications Unspecified
    Reactivity
  • Succinyl-proteome profiling of Dendrobium officinale, an important traditional Chinese orchid herb, revealed involvement of succinylation in the glycolysis pathway
    Year 2017
    Journal BMC GENOMICS
    Authors Feng Shangguo, et al.
    Applications Unspecified
    Reactivity
  • Global analysis of protein lysine succinylation profiles in common wheat
    Year 2017
    Journal BMC GENOMICS
    Authors Zhang Yumei, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability
    Year 2016
    Journal Nature Communications
    Authors Li Lei, et al.
    Applications Unspecified
    Reactivity
  • Succinyl-proteome profiling of a high taxol containing hybrid Taxus species (Taxus × media) revealed involvement of succinylation in multiple metabolic pathways
    Year 2016
    Journal Scientific Reports
    Authors Shen Chenjia, et al.
    Applications Unspecified
    Reactivity
  • First Comprehensive Proteome Analyses of Lysine Acetylation and Succinylation in Seedling Leaves of Brachypodium distachyon L.
    Year 2016
    Journal Scientific Reports
    Authors Zhen Shoumin, et al.
    Applications Unspecified
    Reactivity
  • Crosstalk among proteome, acetylome and succinylome in colon cancer HCT116 cell treated with sodium dichloroacetate
    Year 2016
    Journal Scientific Reports
    Authors Zhu Danxi, et al.
    Applications Unspecified
    Reactivity
  • Lysine Acetylation and Succinylation in HeLa Cells and their Essential Roles in Response to UV-induced Stress
    Year 2016
    Journal Scientific Reports
    Authors Xu Hong, et al.
    Applications Unspecified
    Reactivity
  • Prediction of Protein Lysine Acylation by Integrating Primary Sequence Information with Multiple Functional Features
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Systematic Analysis of the Lysine Succinylome in Candida albicans
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Hailin Zheng, et al.
    Applications Unspecified
    Reactivity
  • Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Dongli He, et al.
    Applications Unspecified
    Reactivity
  • Proteome-Wide Identification of Lysine Succinylation in the Proteins of Tomato (Solanum lycopersicum)
    Year 2016
    Journal PLoS One
    Authors Weibo Jin, et al.
    Applications Unspecified
    Reactivity
  • Quantitative succinylome analysis in the liver of non-alcoholic fatty liver disease rat model
    Year 2016
    Journal Proteome Science
    Authors Cheng Yang, et al.
    Applications Unspecified
    Reactivity
  • Succinylome Analysis Reveals the Involvement of Lysine Succinylation in Metabolism in Pathogenic Mycobacterium tuberculosis
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Mingkun Yang, et al.
    Applications Unspecified
    Reactivity
  • First Succinyl-Proteome Profiling of Extensively Drug-Resistant Mycobacterium tuberculosis Revealed Involvement of Succinylation in Cellular Physiology
    Year 2014
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Longxiang Xie, et al.
    Applications Unspecified
    Reactivity
  • Systematic Identification of the Lysine Succinylation in the Protozoan Parasite Toxoplasma gondii
    Year 2014
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Xiaolong Li, et al.
    Applications Unspecified
    Reactivity
  • Identification of Lysine Succinylation Substrates and the Succinylation Regulatory Enzyme CobB in Escherichia coli*
    Year 2013
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Gozde Colak, et al.
    Applications Unspecified
    Reactivity
  • Lysine Succinylation Is a Frequently Occurring Modification in Prokaryotes and Eukaryotes and Extensively Overlaps with Acetylation
    Year 2013
    Journal Cell Reports
    Authors Brian T. Weinert, et al.
    Applications Unspecified
    Reactivity