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Anti-Succinyllysine Rabbit pAb
Catalog Number: PTM-401
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB IHC-P

Reactivity: All

Synonyms: Ksu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Ksu
UniProt ID

/

Immunogen Succinylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Succinyllysine Rabbit pAb detects proteins post-translationally modified by succinylation on lysine residues. This pan antibody recognizes succinylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
IHC-P 1:100 - 1:500 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Succinylation is a post-translational modification that involves the addition of a succinyl group (-CO-CH2-CH2-CO2 h) is added to a lysine residue of a protein molecule. This modification is found in many proteins, including histones. Although the precise implications of succinylation are under investigation, due to its impact on lysine's charge, transitioning it from +1 to -1 (under physiological pH conditions), and because succinylation introduces a relatively substantial structural moiety with a mass of 100 Da, which is notably larger than the moieties introduced by acetylation (42 Da) or methylation (14 Da), succinylation is anticipated to induce more substantial alterations in both protein structure and function. Key players in the succinylation process comprise 'writers' such as carnitine palmitoyltransferase 1A (CPT1A) and lysine acetyltransferase 2A (KAT2A/GCN5), 'erasers' like sirtuin 5 (SIRT5) and sirtuin 7 (SIRT7), and the pH-dependent reader, the YEATS domain of glioma-amplified sequence 41 (GAS41).

Cellular location

/

Images
Dot Blot

Peptide amount: 4 ng, 20 ng, 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: Succinylated peptide library. Dot 2: Malonylated peptide library.
Dot 3: Glutarylated peptide library. Dot 4: Unmodified peptide library.

WB

Lysates: E. coli, S. cerevisiae, D. melanogaster, mouse, and human cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: (A) PTM-401 + unmodified BSA; (B) PTM-401+ succinylated BSA, 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

IHC-P

Tissue: Human neuroblastoma
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-401.

Research Use

For research use only, not for use in diagnostic procedures.

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References (96)
  • Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner
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  • Lysine 2-Hydroxyisobutyrylation- and Succinylation-Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity
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    Authors Sarah Abdullah, et al.
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  • Histone malonylation is regulated by SIRT5 and KAT2A
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    Authors Ran Zhang, et al.
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  • The Deacylase Sirtuin 5 Reduces Malonylation in Non-mitochondrial Metabolic Pathways in Diabetic Kidney Disease
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    Authors Anuradha Karunanidhi, et al.
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  • Global proteomic analysis reveals lysine succinylation contributes to the pathogenesis of aortic aneurysm and dissection
    Year 2023
    Journal Journal of Proteomics
    Authors Hongwei Zhang, et al.
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  • Acylation of the Rat Brain Proteins is Affected by the Inhibition of Pyruvate Dehydrogenase in vivo
    Year 2023
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    Authors Aleshin Vasily A., et al.
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  • Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection
    Year 2023
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    Authors Yu Zhang, et al.
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  • Altered succinylation of mitochondrial proteins, APP and tau in Alzheimer’s disease
    Year 2022
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    Authors Yang Yun, et al.
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  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
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    Reactivity
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    Year 2021
    Journal Biomolecules
    Authors Artem V. Artiukhov, et al.
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    Reactivity
  • Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome
    Year 2021
    Journal AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
    Authors Jessica N. Peoples, et al.
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    Reactivity
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    Year 2021
    Journal FEBS Open Bio
    Authors Liang Meng, et al.
    Applications Unspecified
    Reactivity
  • Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation
    Year 2021
    Journal CRYOBIOLOGY
    Authors Stuart R. Green, et al.
    Applications Unspecified
    Reactivity
  • Pimozide Alleviates Hyperglycemia in Diet-Induced Obesity by Inhibiting Skeletal Muscle Ketone Oxidation
    Year 2020
    Journal Cell Metabolism
    Authors Rami Al Batran, et al.
    Applications Unspecified
    Reactivity
  • Protein Hyperacylation Links Mitochondrial Dysfunction with Nuclear Organization
    Year 2020
    Journal MOLECULAR CELL
    Authors Smestad John, et al.
    Applications Unspecified
    Reactivity
  • Repression of P53 Function by SIRT5-Mediated Desuccinylation at Lysine 120
    Year 2020
    Journal Cell Reports
    Authors Liu Xing, et al.
    Applications Unspecified
    Reactivity
  • SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching
    Year 2020
    Journal Clinical and Translational Medicine
    Authors Tiantong Ou, et al.
    Applications Unspecified
    Reactivity
  • Lysine-222 succinylation reduces lysosomal degradation of lactate dehydrogenase a and is increased in gastric cancer
    Year 2020
    Journal JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
    Authors Li Xiang, et al.
    Applications Unspecified
    Reactivity
  • Increased protein propionylation contributes to mitochondrial dysfunction in liver cells and fibroblasts, but not in myotubes
    Year 2020
    Journal JOURNAL OF INHERITED METABOLIC DISEASE
    Authors Bart Lagerwaard, et al.
    Applications Unspecified
    Reactivity
  • Impaired mitochondrial medium-chain fatty acid oxidation drives periportal macrovesicular steatosis in sirtuin-5 knockout mice
    Year 2020
    Journal Scientific Reports
    Authors Goetzman Eric S, et al.
    Applications Unspecified
    Reactivity
  • An optimized desuccinylase activity assay reveals a difference in desuccinylation activity between proliferative and differentiated cells
    Year 2020
    Journal Scientific Reports
    Authors Yuan Taolin, et al.
    Applications Unspecified
    Reactivity
  • Nucleotide-binding sites can enhance N-acylation of nearby protein lysine residues
    Year 2020
    Journal Scientific Reports
    Authors James Andrew M, et al.
    Applications Unspecified
    Reactivity
  • Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability
    Year 2020
    Journal AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
    Authors Rongyi Shi, et al.
    Applications Unspecified
    Reactivity
  • Systematic analysis of lysine acetylome and succinylome reveals the correlation between modification of H2A.X complexes and DNA damage response in breast cancer
    Year 2020
    Journal ONCOLOGY REPORTS
    Authors Xiuli Gao, et al.
    Applications Unspecified
    Reactivity
  • Regulation of the α-ketoglutarate dehydrogenasecomplex during hibernation in a small mammal, the Richardson's ground squirrel (Urocitellus richardsonii)
    Year 2020
    Journal BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
    Authors Stuart R. Green, et al.
    Applications Unspecified
    Reactivity
  • A Qualitative Proteome-Wide Lysine Succinylation Profiling of Tea Revealed its Involvement in Primary Metabolism
    Year 2020
    Journal MOLECULAR BIOLOGY
    Authors Qiu C, et al.
    Applications Unspecified
    Reactivity
  • Regulation of UCP1 and Mitochondrial Metabolism in Brown Adipose Tissue by Reversible Succinylation
    Year 2019
    Journal MOLECULAR CELL
    Authors GuoXiao Wang, et al.
    Applications Unspecified
    Reactivity
  • Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI
    Year 2019
    Journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
    Authors Takuto Chiba, et al.
    Applications Unspecified
    Reactivity
  • Targeting oxidative pentose phosphate pathway prevents recurrence in mutant Kras colorectal carcinomas
    Year 2019
    Journal PLOS BIOLOGY
    Authors WenChao Gao, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive Analysis of the Lysine Succinylome and Protein Co-modifications in Developing Rice Seeds *
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Xiaoxi Meng, et al.
    Applications Unspecified
    Reactivity
  • Quantifying Competition among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism
    Year 2019
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Hadi R. Ali, et al.
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    Reactivity
  • Lead-mediated inhibition of lysine acetylation and succinylation causes reproductive injury of the mouse testis during development
    Year 2019
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    Authors Qiangzhen Yang, et al.
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    Reactivity
  • Succinylome Analysis Reveals the Involvement of Lysine Succinylation in the Extreme Resistance of Deinococcus radiodurans
    Year 2019
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    Authors Congli Zhou, et al.
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  • SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense
    Year 2016
    Journal EMBO REPORTS
    Authors Lisha Zhou, et al.
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  • Succinyl-proteome profiling of a high taxol containing hybrid Taxus species (Taxus × media) revealed involvement of succinylation in multiple metabolic pathways
    Year 2016
    Journal Scientific Reports
    Authors Shen Chenjia, et al.
    Applications Unspecified
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  • Sirtuin 5 protects mitochondria from fragmentation and degradation during starvation
    Year 2016
    Journal BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
    Authors Hala Guedouari, et al.
    Applications Unspecified
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  • Prediction of Protein Lysine Acylation by Integrating Primary Sequence Information with Multiple Functional Features
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Yipeng Du, et al.
    Applications Unspecified
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  • Systematic Analysis of the Lysine Succinylome in Candida albicans
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Hailin Zheng, et al.
    Applications Unspecified
    Reactivity
  • Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Dongli He, et al.
    Applications Unspecified
    Reactivity
  • The Role of Mitochondrial Non-Enzymatic Protein Acylation in Ageing
    Year 2016
    Journal PLoS One
    Authors Shin Yee Hong, et al.
    Applications Unspecified
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  • Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p
    Year 2015
    Journal Nature Communications
    Authors Madsen Christian T, et al.
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  • Lysine Malonylation Is Elevated in Type 2 Diabetic Mouse Models and Enriched in Metabolic Associated Proteins *
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Succinylome Analysis Reveals the Involvement of Lysine Succinylation in Metabolism in Pathogenic Mycobacterium tuberculosis
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Mingkun Yang, et al.
    Applications Unspecified
    Reactivity
  • Characterization of the cardiac succinylome and its role in ischemia–reperfusion injury
    Year 2015
    Journal JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
    Authors Jennifer A. Boylston, et al.
    Applications Unspecified
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  • Systematic Identification of the Lysine Succinylation in the Protozoan Parasite Toxoplasma gondii
    Year 2014
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Xiaolong Li, et al.
    Applications Unspecified
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  • Pigment Epithelium-Derived Factor (PEDF) Suppresses IL-1β-Mediated c-Jun N-Terminal Kinase (JNK) Activation to Improve Hepatocyte Insulin Signaling
    Year 2014
    Journal ENDOCRINOLOGY
    Authors Gattu Arijeet K, et al.
    Applications Unspecified
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  • Succinate is an inflammatory signal that induces IL-1β through HIF-1α
    Year 2013
    Journal NATURE
    Authors Tannahill G. M, et al.
    Applications Unspecified
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  • Widespread and Enzyme-independent Nϵ-Acetylation and Nϵ-Succinylation of Proteins in the Chemical Conditions of the Mitochondrial Matrix *♦
    Year 2013
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Gregory R. Wagner, et al.
    Applications Unspecified
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  • Lysine Succinylation Is a Frequently Occurring Modification in Prokaryotes and Eukaryotes and Extensively Overlaps with Acetylation
    Year 2013
    Journal Cell Reports
    Authors Brian T. Weinert, et al.
    Applications Unspecified
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