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Anti-Trimethyl-Histone H3 (Lys9) Rabbit pAb
Catalog Number: PTM-616
$ 340

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB IHC-P ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: H3K9me3

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K9me3
UniProt ID

P68431

Immunogen Trimethylated human histone H3 (Lys9) peptide
MW (kDa) 15
Specificity Anti-Trimethyl-Histone H3 (Lys9) Rabbit pAb detects histone H3 when it is trimethylated at Lys9.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human, Mouse, Rat
IHC-P 1:500 - 1:1000 Human, Mouse
ICC/IF 1:200 - 1:1000 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone methylation primarily occurs at lysine and arginine residues on the amino terminus of core histones. The methylation of histones can either enhance or repress gene transcription, depending on the specific amino acids (Lys or Arg) being methylated and the number of attached methyl groups (mono-, di-, or tri-methylation for Lys; mono-di-symmetric/asymmetric methylation for Arg). Lysine methylation is commonly observed at histone H3 Lys residues 4, 9, 27, 36, 79, and histone H4 Lys20, while Arginine methylation predominantly occurs at histone H3 Arg residues 2, 8, 17, 26, and histone H4 Arg3. The regulation of histone methylation is primarily governed by histone methyltransferases (HMTs) and histone demethylases (HDMs).

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K9 trimethyl. Lane 2: H3K4 monomethyl.
Lane 3: H3K4 dimethyl. Lane 4: H3K4 trimethyl.
Lane 5: H3K9 monomethyl. Lane 6: H3K9 dimethyl.
Lane 7: H3K14 monomethyl. Lane 8: H3K9 unmodified.

WB

Lysates: 1. HeLa cells; 2. C2C12 cells; 3. COS cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 17 kDa

IHC-P

Tissue: Human spleen
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-616.

Tissue: Mouse stomach
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-616.

ICC/IF

Samples: MCF-7 cells
Fixative: 4% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-616.

Research Use

For research use only, not for use in diagnostic procedures.

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