/ EN
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
/ EN
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant
Anti-TTK Mouse mAb
Catalog Number: PTM-7030
$ 280

Clone Number: JMMR-3601

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: MPS1, MPS1L1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms MPS1, MPS1L1
UniProt ID

P33981

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

TTK also named as Mps1 or PYT, is a cell cycle regulated dual specificity kinase present in rapidly proliferating tissues and cell lines. TTK localizes to kinetochores and centromeres and is an essential component of the mitotic spindle checkpoint as well as centrosome duplication. The mitotic checkpoint inhibits entry into anaphase until all chromosomes are attached to the spindle; inhibition of this process leads to genomic instability and tumorigenesis. Phosphorylation of the BLM helicase at Ser144 by TTK maintains chromosome stability during mitosis. Small molecule inhibitors of TTK can block the spindle checkpoint response, thereby making TTK a potential therapeutic target.

Cellular location

Cytoskeleton, Nucleus, Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: HeLa, 2: Ramos
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 97 kDa
Observed band size: 100 kDa

Research Use

For research use only, not for use in diagnostic procedures.