/ EN
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
/ EN
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-Ubiquitin Rabbit mAb (N-terminal, BSA and Azide Free)
Catalog Number: PTM-1124RM
$ 465

Clone Number: PDPTM-O0402

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: All

Synonyms: ubiquitin

Product Size
100 μg
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms ubiquitin
UniProt ID

P62979

Immunogen Synthetic peptide corresponding to the N-terminus of human ubiquitin
MW (kDa) Multiple
Specificity Anti-Ubiquitin Rabbit mAb (BSA and Azide Free) detects endogenous ubiquitin, polyubiquitin, and ubiquitinated proteins.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 All
Properties
Purity Protein A purified
Constituents PBS
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs). The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human). This modification triggers a “trans-histone” modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity.

Cellular location

Cytoplasm, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 4℃ overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysates: 1: HeLa, 2: MCF-7, 3: BRL, 4: NIH-3T3
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: Multiple
Observed MW: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
  • View More Images(3)
    $ 300
    Anti-Ubiquitin Rabbit pAb (N-terminal)
    Catalog #
    PTM-1106
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat, Monkey
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(2)
    $ 280
    Recombinant Rabbit Monoclonal
    Anti-Ubiquitin Rabbit mAb (N-terminal)
    Catalog #
    PTM-1106RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(2)
    $ 290
    Anti-Ubiquitin Mouse mAb (N-terminal)
    Catalog #
    PTM-1107
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(2)
    $ 270
    Recombinant
    Anti-Ubiquitin Mouse mAb (N-terminal)
    Catalog #
    PTM-5798
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(7)
    $ 280
    Recombinant Rabbit Monoclonal
    Anti-Ubiquitin Rabbit mAb (N-terminal)
    Catalog #
    PTM-7311
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(4)
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(5)
    $ 280
    Recombinant Rabbit Monoclonal
    Anti-SQSTM1/p62 Rabbit mAb
    Catalog #
    PTM-5483
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(1)
    $ 2,110
    Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-1104
    Host
    /
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images(2)
    $ 290
    Anti-Ubiquitin Rabbit pAb (C-terminal)
    Catalog #
    PTM-1105
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat, Monkey
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P