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Recombinant Rabbit Monoclonal
Anti-Ubiquitin Rabbit mAb (N-terminal)
Catalog Number: PTM-1106RM
References (25)
$ 280

Clone Number: 14H4L1

Host: Rabbit Clonality: Recombinant monoclonal

Applications： WB ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: /

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms /
UniProt ID

P62979

Immunogen Synthetic peptide corresponding to the N-terminus of human ubiquitin
MW (kDa) Multiple
Specificity Anti-Ubiquitin Rabbit mAb (N-terminal) detects endogenous ubiquitin, polyubiquitin, and ubiquitinated proteins.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ICC/IF 1:50 - 1:200 Human, Mouse
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation, modulates membrane protein trafficking, alters protein-protein interactions, and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminal Gly76 and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule, resulting in polyubiquitin chains with different functions. Lys6-linked may be involved in DNA repair; Lys11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys29-linked is involved in lysosomal degradation; Lys33-linked is involved in kinase modification; Lys48-linked is involved in protein degradation via the proteasome; Lys63-linked is involved in endocytosis, and DNA-damage responses.

Cellular location

Nucleus, Cytoplasm

Images
WB

Lysate: HeLa, MCF-7, BRL, NIH/3T3
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: Multiple
Observed band size: Multiple
ICC/IF

Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:200
Primary Ab incubation: overnight at 4°C
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counterstain: DAPI (blue)
Description: The green color represents the positive signal observed with PTM-1106RM.

Cell line: NIH/3T3
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:200
Primary Ab incubation: overnight at 4°C
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counterstain: DAPI (blue)
Description: The green color represents the positive signal observed with PTM-1106RM.

Research Use

For research use only, not for use in diagnostic procedures.

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References (25)
  • RNF126 mediates fetal growth restriction via ubiquitination-dependent degradation of the MYH9/MYH10 complex
    Year 2026
    Journal iScience
    Authors Lilin Lu, et al.
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  • Arabidopsis CIRP1 E3 ligase modulates drought and oxidative stress tolerance and reactive oxygen species homeostasis by directly degrading catalases
    Year 2025
    Journal J Integr Plant Biol
    Authors Heng Yang, et al.
    Applications Unspecified
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  • Comparative ubiquitinome analysis reveals the roles of protein ubiquitination in the heat stress response from Metarhizium robertsii
    Year 2025
    Journal Appl Environ Microbiol
    Authors Jueping Song, et al.
    Applications WB
    Reactivity
  • Deubiquitylases MoUbp12 and MoUbp14 modulated by transcription factor MoMsn2 are critical for mitochondrial fusion/fission balance and infectious growth of the rice blast fungus
    Year 2025
    Journal Phytopathol Res
    Authors Xingyu Wang, et al.
    Applications Unspecified
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  • O-GlcNAcylated Hsp47 as a predictive biomarker in colorectal cancer: Kaempferol targets OGT-collagen axis for therapeutic intervention
    Year 2025
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    Authors Chishun Zhou, et al.
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  • Risk stratification and prognosis determination based on protein lysine crotonylation in patients with resected pancreatic cancer: A multicenter study
    Year 2025
    Journal Clin Cancer Res
    Authors Lianjing Cao, et al.
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  • SDCCAG3 inhibits adipocyte hypertrophy and improves obesity-related metabolic disorders via SDCCAG3/SMURF1/PPARγ axis
    Year 2025
    Journal J Lipid Res
    Authors Fenglei Huo, et al.
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  • Sorafenib promotes the E3 ubiquitin ligase FBXW7 to increase tau degradation and ameliorate tauopathies
    Year 2025
    Journal Acta Pharm Sin B
    Authors Yunqiang Zhou, et al.
    Applications Unspecified
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  • Streptococcus mutans regulates ubiquitin modification of Candida albicans in the bacterial-fungal interaction
    Year 2025
    Journal PLoS Pathog
    Authors Yixin Zhang, et al.
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  • The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan
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    Journal Plant Physiol
    Authors Xueying Zhang, et al.
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  • Ubiquitin-mediated degradation restricts spatiotemporal accumulation of the cytoplasmic male sterility protein WA352 to anthers in rice
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  • Deubiquitylase OTUD3 regulates integrated stress response to suppress progression and sorafenib resistance of liver cancer
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  • Hypoxia exposure induces lactylation of Axin1 protein to promote glycolysis of esophageal carcinoma cells
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  • Mechanistic role of RND3-regulated IL33/ST2 signaling on cardiomyocyte senescence
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  • O-GlcNAcylation of YTHDF2 antagonizes ERK-dependent phosphorylation and inhibits lung carcinoma
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  • Tenovin-6 exhibits inhibitory effects on the growth of Sonic Hedgehog (SHH) medulloblastoma, as evidenced by both in vitro and in vivo studies
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  • The role of MrUbp4, a deubiquitinase, in conidial yield, thermotolerance, and virulence in Metarhizium robertsii
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    Journal J Invertebr Pathol
    Authors Hongzhi Zhang, et al.
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  • Ketogenic diet reshapes cancer metabolism through lysine β-hydroxybutyrylation
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    Journal Nat Metab
    Authors Junhong Qin, et al.
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  • FBXO7 ubiquitinates PRMT1 to suppress serine synthesis and tumor growth in hepatocellular carcinoma
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    Journal Nat Commun
    Authors Li Luo, et al.
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  • Trichinella spiralis inhibits myoblast differentiation by targeting SQSTM1/p62 with a secreted E3 ubiquitin ligase
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    Journal iScience
    Authors Jian da Pang, et al.
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    Reactivity
  • Global proteomic analysis reveals lysine succinylation contributes to the pathogenesis of aortic aneurysm and dissection
    Year 2023
    Journal J Proteomics
    Authors Hongwei Zhang, et al.
    Applications Unspecified
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  • HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition
    Year 2023
    Journal Cell Biol Int
    Authors Gang Nie, et al.
    Applications Unspecified
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  • O-GlcNAc has crosstalk with ADP-ribosylation via PARG
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    Journal J Biol Chem
    Authors Jie Li, et al.
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  • Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection
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    Journal PeerJ
    Authors Yu Zhang, et al.
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