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Recombinant Rabbit Monoclonal

Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb

Catalog Number: PTM-7449

$ 330

Clone Number: JMMR-N1208

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF IP ChIP

Reactivity: Human, Mouse

Synonyms: H2AK119ub

Product Size

100 μl

Shipping: Ambient temperature

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General Information

Isotype
Conjugate	Unconjugated
Synonyms	H2AK119ub
UniProt ID	P04908
Immunogen	Ubiquitinated human histone H2A (Lys119) peptide
MW (kDa)
Specificity

Product Usage Information

Applications	Dilution	Recommended Species

Properties

Purity
Constituents	PBS, Glycerol, BSA
Storage	Store at -20°C. Avoid freeze/thaw cycles.
Stability	Stable for 12 months from date of receipt/reconstitution.

Target Information

Background

Cellular location

Images

WB
	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: NIH-3T3, 3: C2C12 Protein loading amount: 20 μg Exposure time: 3 s Predicted MW: 23 kDa Observed MW: 23 kDa

ICC/IF
	Cell line: HeLa Fixation: 4% PFA Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:100 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red) Comment: P

IP
	P of HeLa cells extracts: IP Ab incubation condition: PTM-7449, 4°C overnight, 1:100 dilution WB primary Ab incubation condition: PTM-7449, room temperature 2h , 1:1000 dilution Secondary Ab: Anti-Rabbit IgG for IP (HRP) Blocking buffer: 5% NFDM/TBST Dilution buffer: 5% NFDM/TBST 1: 5%% Input 2: IP with Rabbit monoclonal IgG Isotype 3: IP with PTM-7449 Observed MW: 23 kDa Exposure time: 30 s

Research Use

For research use only, not for use in diagnostic procedures.