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Anti-Ubiquityl-Histone H2A (Lys119) Rabbit pAb
Catalog Number: PTM-1119
$ 340

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: H2AK119ub

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2AK119ub
UniProt ID

P04908

Immunogen Ubiquitinated human histone H2A (Lys119) peptide
MW (kDa) 23
Specificity Anti-Ubiquityl-Histone H2A (Lys119) Rabbit pAb detects histone H2A only when it is ubiquitinated at Lys119. This antibody has been shown to selectively recognize ubiquitinated H2A peptide at Lys119, but not the acetylated, crotonylated, or unmodified H2A peptide at Lys119 or Lys118.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds
The list of peptides used in the experiment is provided in the table below.
WB

Lysate: HeLa, N2a, BRL, mouse liver
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 23 kDa
Observed band size: 23 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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