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Recombinant Rabbit Monoclonal
Anti-Von Willebrand Factor Rabbit mAb
Catalog Number: PTM-7086
$ 280

Clone Number: JRMR-10117(2)-19

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human, Mouse

Synonyms: vWF, F8VWF, VWD, Von Willebrand antigen II

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms vWF, F8VWF, VWD, Von Willebrand antigen II
UniProt ID

Q8CIZ8

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:5000 - 1:10000 Mouse
IHC-P 1:100 - 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

VWF (Von Willebrand factor) is a multimeric plasma glycoprotein that promotes adhesion of platelets to sites of vascular injury. Mature circulating VWF is made up of disulfide-bonded multimers that are in a complex with factor VIII. VWF is stored in secretory Weibel-Palade bodies in endothelial cells. It is synthesized as a large precursor protein and undergoes extensive posttranslational modifications including dimerization in the endoplasmic reticulum followed by cleavage of the pro-peptide and multimerization in the Golgi apparatus. VWF is important in hemostasis, and genetic defects in the structure and modification of VWF can cause von Willebrand disease (VWD), the most common congenital bleeding disorder in humans.  Alternatively, increased levels of VWF have been shown to be involved in acute coronary thrombosis and are a clinical risk marker for atherosclerosis.

Cellular location

Secreted

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: Mouse platelet
Protein loading amount: 20 μg
Exposure time: 60 seconds
Predicted band size: 309 kDa
Observed band size: 309 kDa

IHC-P

Tissue: Human tonsil
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-7086

Tissue: Human breast carcinoma
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-7086

Research Use

For research use only, not for use in diagnostic procedures.