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Recombinant
Anti-Ku80 Mouse mAb
Catalog Number: PTM-5405
$ 280

Clone Number: 10647

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB ICC/IF

Reactivity: Human

Synonyms: XRCC5, G22P2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms XRCC5, G22P2
UniProt ID

P13010

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:200 - 1:1000 Human
ICC/IF 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Ku80 is a protein that, in humans, is encoded by the XRCC5 gene. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 4°C overnight
Lysate: HeLa, Jurkat, A549
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 86 kDa
Observed band size: 86 kDa

ICC/IF

Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-5405

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 290
    Recombinant Rabbit Monoclonal
    Anti-Ku80 Rabbit mAb
    Catalog #
    PTM-5279
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Mouse
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis
    Year 2024
    Journal MOLECULAR CELL
    Authors Kaiwen Bao, et al.
    Applications Unspecified
    Reactivity