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Recombinant Rabbit Monoclonal
Anti-β-Hydroxybutyryllysine Rabbit mAb
Catalog Number: PTM-1201RM
$ 435

Clone Number: 7H1L3

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: All

Synonyms: Kbhb

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kbhb
UniProt ID

/

Immunogen β-Hydroxybutylated lysine peptides
MW (kDa) Multiple
Specificity Anti-β-Hydroxybutyllysine Rabbit mAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 All
IHC-P 1:200 - 1:1000 Human
ICC/IF 1:100 - 1:200 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.

Cellular location

/

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds
The list of peptides used in the experiment is provided below.
Lane 1: β-Hydroxybutyryllysine peptide library. Lane 2: Acetyllysine peptide library.
Lane 3: Propionyllysine peptide library. Lane 4: Butyryllysine peptide library.
Lane 5: Crotonyllysine peptide library. Lane 6: 2-Hydroxyisobutyryllysine peptide library.
Lane 7: Unmodified peptide library.
WB

Lysates: (-): HeLa cells; (+): HeLa cells + sodium 3-hydroxybutyrate (50 mM, 72 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: Multiple
Observed band size: Multiple
IHC-P

Tissue: Human kidney
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Comment: The brown color represents the positive signal observed with PTM-1201RM.

Tissue: Human placenta
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Comment: The brown color represents the positive signal observed with PTM-1201RM.
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells + sodium 3-hydroxybutyrate (100 mM, 72 hours)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:200
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Comment: The green color represents the positive signal observed with PTM-1201RM.

Research Use

For research use only, not for use in diagnostic procedures.

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References (31)
  • TIGAR maintains intestinal epithelial regeneration by stabilizing HMGCL and promoting β-catenin β-hydroxybutyrylation in burn-induced sepsis
    Year 2026
    Journal Cell Death & Disease
    Authors Zhang Panyang, et al.
    Applications Unspecified
    Reactivity
  • Ketogenic diet dampens excitatory neurotransmission by shrinking synaptic vesicle pools
    Year 2026
    Journal Cell Reports
    Authors Marion I. Stunault, et al.
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  • Comprehensive Proteomics and β-Hydroxybutyrylation Profiling in Starvation-Induced Gastrocnemius Muscle Remodeling
    Year 2026
    Journal Biology-Basel
    Authors Leilei Cui, et al.
    Applications Unspecified
    Reactivity
  • Enhancing the structural stability of P29-targeted monoclonal antibodies via β-hydroxybutyrylation modification improves their therapeutic performance in alveolar echinococcosis
    Year 2026
    Journal Frontiers in Cellular and Infection Microbiology
    Authors Shiqin Yuan, et al.
    Applications Unspecified
    Reactivity
  • β-Hydroxybutyrate ameliorates lipopolysaccharide-induced liver injury through β-hydroxybutyrylation of the SOD2 protein in mice
    Year 2025
    Journal Redox Biology
    Authors Ya-Ping Bai, et al.
    Applications Unspecified
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  • TAF1 acetyltransferase promotes colorectal carcinoma metastasis by catalyzing β-hydroxybutyrylation of KCTD9
    Year 2025
    Journal ONCOGENE
    Authors Su Jun, et al.
    Applications Unspecified
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  • HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma
    Year 2025
    Journal Cell Death & Disease
    Authors He Jiang, et al.
    Applications Unspecified
    Reactivity
  • β-Hydroxybutyrate enhances malate dehydrogenase 2 β-hydroxybutyrylation to alleviate hepatic steatosis in MASLD
    Year 2025
    Journal CELLULAR AND MOLECULAR LIFE SCIENCES
    Authors Cai Jingshu, et al.
    Applications Unspecified
    Reactivity
  • Modulating Lysine Crotonylation in Ulcerative Colitis Maintains Mitochondrial Homeostasis
    Year 2025
    Journal Exploration
    Authors Tongtong Liu, et al.
    Applications Unspecified
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  • β-hydroxybutyrate dehydrogenase promotes pancreatic cancer cell proliferation through regulation of the NAD+/NADH balance and mitochondrial acetylation
    Year 2025
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Xiujuan Wei, et al.
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  • Β-Hydroxybutyrate inhibits centriole duplication and mitochondrial dysfunction through β-hydroxybutyrylation of ANXA11 in diabetic cardiomyopathy rats
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    Journal CELLULAR SIGNALLING
    Authors Jingyu Liu, et al.
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  • Regulation of plant immunity through histone H3 β-hydroxybutyrylation-mediated transcriptional control
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    Journal Nature Communications
    Authors Xu Qiutao, et al.
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  • Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells
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    Journal ACS Chemical Biology
    Authors Xinlong Guo, et al.
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  • Crotonylation driving Streptococcus pneumoniae adaption and virulence
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    Journal Journal of Advanced Research
    Authors Nan Li, et al.
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  • Altered immunometabolic response to fasting in humans living with obesity
    Year 2025
    Journal iScience
    Authors Helena Neudorf, et al.
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  • D-β-hydroxybutyrate upregulates tight junction protein zonula occludens-1 attenuating lipopolysaccharide-stimulated cerebral microvascular hyperpermeability
    Year 2025
    Journal TISSUE & CELL
    Authors Xinhao Li, et al.
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  • Resveratrol attenuates the CoCl2-induced hypoxia damage by regulation of lysine β-hydroxybutyrylation in PC12 cells
    Year 2025
    Journal BMC Neurology
    Authors Wang Yamei, et al.
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  • β-hydroxybutyrate serves as a regulator in ketone body metabolism through lysine β-hydroxybutyrylation
    Year 2025
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Jie Fang, et al.
    Applications Unspecified
    Reactivity
  • Reversible histone deacetylase activity catalyzes lysine acylation
    Year 2025
    Journal Nature Chemical Biology
    Authors Tsusaka Takeshi, et al.
    Applications Unspecified
    Reactivity
  • β-hydroxybutyrate facilitates mitochondrial-derived vesicle biogenesis and improves mitochondrial functions
    Year 2025
    Journal MOLECULAR CELL
    Authors Min Tang, et al.
    Applications Unspecified
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  • β-hydroxybutyrylation and O-GlcNAc modifications of STAT1 modulate antiviral defense in aging
    Year 2025
    Journal Cellular & Molecular Immunology
    Authors Zuo Yibo, et al.
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  • β-Hydroxybutyrate suppresses M1 macrophage polarization through β-hydroxybutyrylation of the STAT1 protein
    Year 2024
    Journal Cell Death & Disease
    Authors Bai Ya-Ping, et al.
    Applications Unspecified
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  • Rapid modulation of interscapular brown adipose tissue mitochondrial activity by ketosis induced by 1,3‐butanediol administration to rats
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  • Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis
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    Authors Li Yinzheng, et al.
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    Authors Zhang Yifan, et al.
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  • Sodium benzoate induces pancreatic inflammation and β cell apoptosis partially via benzoylation
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    Year 2022
    Journal Journal of Proteomics
    Authors Ni Yang, et al.
    Applications Unspecified
    Reactivity
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