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Recombinant Rabbit Monoclonal
Anti-β-Hydroxybutyryllysine Rabbit pAb
Catalog Number: PTM-1201
This item is discontinued.
Please see alternative products below.

Clone Number: /

Host: Rabbit Clonality: Polyconal

Applications: WB ICC/IF

Reactivity: All

Synonyms: Kbhb

Product Size
100μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

  • View More Images(5)
    $ 435
    Recombinant Rabbit Monoclonal
    Anti-β-Hydroxybutyryllysine Rabbit mAb
    Catalog #
    PTM-1201RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kbhb
UniProt ID

/

Immunogen β-Hydroxybutylated lysine peptides
MW (kDa) Multiple
Specificity Anti-β-Hydroxybutyllysine Rabbit pAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
ICC/IF 1:50 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.

Cellular location

/

Images
Dot Blot

Peptide/protein amount: 10 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided in the table below.

WB

Lysates: HeLa, C2C12, COS, (-): HeLa, (+): HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugated)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

ICC/IF

Samples: (A) HeLa; (B) HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Description: The green color represents the positive signal observed with PTM-1201.

Research Use

For research use only, not for use in diagnostic procedures.

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References (6)
  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
    Year 2022
    Journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
    Authors Lin Dang, et al.
    Applications Unspecified
    Reactivity
  • β-hydroxybutyrate reduces reinstatement of cocaine conditioned place preference through hippocampal CaMKII-α β-hydroxybutyrylation
    Year 2022
    Journal Cell Reports
    Authors Hongchun Li, et al.
    Applications Unspecified
    Reactivity
  • The regulatory enzymes and protein substrates for the lysine β-hydroxybutyrylation pathway
    Year 2021
    Journal Science Advances
    Authors He Huang, et al.
    Applications Unspecified
    Reactivity
  • Ketogenesis impact on liver metabolism revealed by proteomics of lysine β-hydroxybutyrylation
    Year 2021
    Journal Cell Reports
    Authors Kevin B. Koronowski, et al.
    Applications Unspecified
    Reactivity
  • The Leucine Catabolite and Dietary Supplement β-Hydroxy-β-Methyl Butyrate (HMB) as an Epigenetic Regulator in Muscle Progenitor Cells
    Year 2021
    Journal Metabolites
    Authors Virve Cavallucci, et al.
    Applications Unspecified
    Reactivity
  • Ketogenesis-generated β-hydroxybutyrate is an epigenetic regulator of CD8+ T-cell memory development
    Year 2019
    Journal NATURE CELL BIOLOGY
    Authors Zhang Huafeng, et al.
    Applications Unspecified
    Reactivity