Clone Number: /
Host: Rabbit Clonality: Polyconal
Applications: WB ICC/IF
Reactivity: All
Synonyms: Kbhb
Shipping: Ambient temperature
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Isotype | IgG |
Conjugate | Unconjugated |
Synonyms | Kbhb |
UniProt ID | |
Immunogen | β-Hydroxybutylated lysine peptides |
MW (kDa) | Multiple |
Specificity | Anti-β-Hydroxybutyllysine Rabbit pAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences. |
Applications | Dilution | Recommended Species |
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WB | 1:500 - 1:1000 | All |
ICC/IF | 1:50 | Human |
Purity | Protein A and immunogen affinity purified |
Constituents | PBS, Glycerol, BSA |
Storage | Store at -20°C. Avoid freeze/thaw cycles. |
Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.
Cellular location
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Dot Blot | |
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Peptide/protein amount: 10 ng |
WB | |
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Lysates: HeLa, C2C12, COS, (-): HeLa, (+): HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) |
ICC/IF | |
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Samples: (A) HeLa; (B) HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) |
Research Use
For research use only, not for use in diagnostic procedures.