/ EN
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
/ EN
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Goat Anti-Mouse IgG H&L (HRP Conjugate)
Catalog Number: PTM-7168
References (4)
$ 135

Clone Number: /

Host: Goat Clonality: Polyclonal

Applications: WB IHC-P

Reactivity: Mouse

Synonyms: /

Product Size
1ml
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate HRP
Synonyms /
UniProt ID

/

Immunogen Mouse IgG
MW (kDa) 50
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:20000 Mouse
IHC-P 1:50 - 1:100 Mouse
Properties
Purity Immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Immunogen is mouse IgG whole molecule

Cellular location

/

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: PTM-7168
Secondary Ab dilution: 1:20000/ 1:50000
Secondary Ab incubation: 45 minutes at room temperature
Lysate: Jurkat
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 50 kDa
Observed MW: 50 kDa

IHC-P

Tissue: Human kidney
Section type: Formalin-fixed & paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour atroom temperature
Secondary Ab: PTM-7168
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signalfor PTM-6773

Tissue: Human seminoma
Section type: Formalin-fixed & paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour atroom temperature
Secondary Ab: PTM-7168
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signalfor PTM-6831

Tissue: Rat kidney
Section type: Formalin-fixed & paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour atroom temperature
Secondary Ab: PTM-7168
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signalfor PTM-6042

Research Use

For research use only, not for use in diagnostic procedures.

References (4)
  • Replacement of the Loop 4 region in CPV VP2 with corresponding PPV segments affects N-terminal cleavage, VLP morphology, hemagglutination, and cell binding
    Year 2026
    Journal Vet Microbiol
    Authors Shengke Chang, et al.
    Applications Unspecified
    Reactivity
  • G9a epigenetically suppresses CXCL10 expression and inhibits anti-tumor immunity in hepatocellular carcinoma
    Year 2025
    Journal Int J Cancer
    Authors Xinxin Chai, et al.
    Applications Unspecified
    Reactivity
  • PSAT1 knockdown mediated VDAC1 oligomerization promotes ferroptosis and suppresses cell proliferation in esophageal squamous cell carcinoma
    Year 2025
    Journal Int J Biol Macromol
    Authors Ruili Ren, et al.
    Applications Unspecified
    Reactivity
  • Acetylation of K188 and K192 inhibits the DNA-binding ability of NarL to regulate Salmonella virulence
    Year 2023
    Journal Appl Environ Microbiol
    Authors Liu-Qing Zhang, et al.
    Applications Unspecified
    Reactivity