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Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Catalog Number: PTM-6261
$ 135

Clone Number: /

Host: Goat Clonality: Polyclonal

Applications: WB IHC-P

Reactivity: Rabbit

Synonyms:

Product Size
1 ml
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype /
Conjugate HRP
Synonyms
UniProt ID

/

Immunogen Rabbit IgG
MW (kDa) /
Specificity Goat Anti-Rabbit IgG H&L pAb pAb (HRP Conjugate) is conjugated to horseradish peroxidase (HRP Conjugate) for chemiluminescent detection as a secondary antibody. The antibody recognizes heavy chain and light chain of rabbit IgG.
Product Usage Information
Applications Dilution Recommended Species
ELISA 1:2000 - 1:10000 Rabbit
WB 1:2000 - 1:10000 Rabbit
IHC-P 1:50 - 1:100 Rabbit
Properties
Purity Immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Immunoglobulin G (IgG) is the predominant immunoglobulin class present in rabbit serum, and is also the most common isotype in rabbit polyclonal and monoclonal research antibodies. Unlike mouse and rat IgG which have multiple subclasses, rabbit IgG only contains one single subclass. A typical IgG is composed of two identical heavy chains of 50 kDa each joined by disulfide bonds to two light chains of approximately 25 kDa.

Cellular location

Secreted

Images
WB

Lysate: HeLa cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab: PTM-5442, Anti-alpha Tubulin Rabbit mAb
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: PTM-6261, Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Secondary Ab dilution: 1:10000
Secondary Ab incubation condition: 45 minutes at room temperature
Exposure time: 10 seconds
Predicted band size: 50 kDa
Observed band size: 50 kDa

IHC-P

Tissue: Human kidney
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab: PTM-5376, Anti-Vimentin Rabbit mAb
Secondary Ab dilution: 1:100
Secondary Ab incubation condition: 30 minutes at room temperature
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with secondary antibody PTM-6261.

Tissue: Mouse kidney
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab: PTM-5376, Anti-Vimentin Rabbit mAb
Secondary Ab dilution: 1:100
Secondary Ab incubation condition: 30 minutes at room temperature
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with secondary antibody PTM-6261.

Tissue: Rat kidney
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab: PTM-5376, Anti-Vimentin Rabbit mAb
Secondary Ab dilution: 1:100
Secondary Ab incubation condition: 30 minutes at room temperature
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with secondary antibody PTM-6261.

Research Use

For research use only, not for use in diagnostic procedures.

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    Conjugate
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    Reactivity
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    Applications:
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