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The figure shows the overall situation of the identified peptides and proteins filtered in the database search results.
The figure shows the length distribution of peptides identified by mass spectrometry.Most of the peptides were distributed in 7-20 amino acids, which conformed to the general rules based on enzymatic digestion and mass spectrometry fragmentation.
The figure shows the distribution of peptide number of proteins. Most proteins correspond to more than two peptides. During quantification, a protein corresponding to multiple specific peptides (or corresponding to multiple spectra) is beneficial to increase the accuracy and reliability of the quantitative results.
The MaxQuant computational platform for mass spectrometry-based shotgun proteomics-2016-Nature Protocols
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