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PTM BIO recently launched a leading generation, full coverage, high-precision targeted proteomics product-4D-PRM, providing a new and powerful tool for targeted protein validation and helping scientists in their work. 4D-PRM technology, combined with trapped Ion mobility spectrometry (TIMS) for 4D separation, improves the selectivity and accuracy of quantification compared with ordinary PRM, and combines PASEF high scanning speed to greatly improve the throughput, achieving full coverage and more accurate target proteomics analysis.
4D-PRM leverages the power of the 4D platform's unique trapped ion mobility technology (TIMS) to perform fourth-dimensional separation, resulting in enhanced selectivity and sensitivity of peptide ions compared to conventional PRM. Furthermore, by combining this approach with PASEF's fast scanning speed, 4D-PRM can detect a higher number of target ions, leading to more comprehensive coverage and improved accuracy in targeted proteomics verification. This breakthrough technology opens up exciting new possibilities in the field of targeted proteomics research.
1. Full coverage of differential protein verification：
The 4D-PRM method enables PRM detection of over 200 differential proteins in a short liquid phase gradient, achieving over 80% increase in protein group. The method can even effectively quantify proteins with low intensity rankings in the proteome results, showcasing its high sensitivity. As a result, 4D-PRM provides an efficient and reliable tool for targeted proteomics research, offering robust and accurate protein quantification even for low-abundance proteins.
2. Quantification is more accurate：
The 4D-PRM provides an enhanced level of ion selectivity. The inclusion of ion mobility as an additional dimension enables the technology to effectively differentiate ions with the same mass-to-charge ratio but different shapes and cross-sections, resulting in improved selectivity. Furthermore, the rapid scanning capability allows for the collection of more data points, significantly enhancing the accuracy. These features make 4D-PRM a powerful tool for targeted proteomics analysis.
4D-PRM addresses the limitations of traditional PRM by offering exceptional sensitivity, scanning speed, and selectivity, as well as high reproducibility and precision in quantification, even in applications with high targeted ion counts. In addition to the validation of differential target proteins in conventional proteomic studies, 4D-PRM enables reliable quantitative analysis of potential candidate biomarkers, unlocking new opportunities for targeted proteomics techniques in large-scale biomarker validation and clinical translational applications. With its numerous benefits, 4D-PRM represents a significant advance in targeted proteomics, enabling more accurate and efficient analysis samples.
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