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Proteomics analysis at the single-cell level using mass spectrometry (MS) has high research and application value, as protein is the main driving factor of cell function. Single-cell proteomics can fill the gap of single-cell genomics and transcriptomics in detecting protein abundance, post-transcriptional regulation, and post-translational modification. Moreover, single-cell proteomic analysis provides accurate information for the study of cell heterogeneity, the construction of fine protein molecular maps, and the analysis of spatial protein networks in biological systems. It offers effective research methods for various areas such as tumor heterogeneity, stem cell differentiation, and germ cell development.
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