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Exosomes are membranous vesicles secreted by cells with a diameter usually ranging from 30-150 nm. They are present in various body fluids and contain various biological molecules, such as proteins, lipids, and nucleic acids (including miRNAs and ncRNAs). Recent research has revealed that exosomes form a unique intercellular communication system that affects cellular physiology and is closely associated with the development of many diseases, particularly cancer. Proteomics, particularly mass spectrometry-based high-throughput proteomics, is an essential tool for studying exosomes. PTM BIO provides high-quality identification and quantitative analysis of protein composition in exosomes using a high-throughput and high-precision protein mass spectrometry platform and an experienced informatics analysis platform.
The qEV size exclusion method is used for exosome isolation in PTM BIO:
1. qEV is capable of processing multiple samples simultaneously, providing high stability in exosome enrichment, enabling rapid separation of large-scale clinical samples.
2. The combined use of transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) for characterization ensures high-purity exosomes, consistent with the "MISEV2018" guideline for exosome research.
3. The Blood+DIA analytical strategy enhances the exosome proteome identification.
4. The detection of high-purity exosome proteins includes the majority of exosome-positive proteins (as reported by Raghu Kalluri et al. in Nature Cell Biology, 2021).
As research progresses, exosomes have been found to play a vital biological role in several areas including cancer, immunity, stem cells, cardiovascular and brain diseases, as well as COVID-19. Exploring their protein components is of great significance in understanding their molecular mechanisms and discovering new biomarkers.
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